Journal: International Journal of Nanomedicine

Article Title: Iron oxide magnetic nanoparticles combined with actein suppress non-small-cell lung cancer growth in a p53-dependent manner

doi: 10.2147/IJN.S127549

Figure Lengend Snippet: Fe 3 O 4 MNPs and AT induced apoptosis in NSCLC cells through caspase 3 activation. Notes: ( A ) Left: A549 cells were given AT, MNPs, or a combination of the two at the concentrations indicated for 24 hours. Total protein was extracted and subjected to immunoblot analysis for cleaved caspase 8, caspase 9, and caspase 3 detection. GAPDH was used as loading control. Right: quantification of Western blot analysis. ( B ) Left: H1975 cells were treated with AT, MNPs, or AT-MNP combination for 24 hours. Whole-cell protein was extracted and examined via immunoblot analysis for cleaved caspase 8, caspase 9 and caspase 3 determination. GAPDH was used as loading control. Right: quantification of Western blot analysis. ( C ) Caspase 3 (left) and caspase 9 (right) activation was determined in A549 cells administered AT and MNPs alone or a combination thereof in the absence or presence of caspase 3 or caspase 9 inhibitor for 24 hours. ( D ) Caspase 3 (left) and caspase 9 (right) activation was determined in H1975 cells treated with AT and MNPs alone or a combination thereof in the absence or presence of caspase 3 or caspase 9 inhibitor for 24 hours. Values are expressed as means ± standard error of mean. * P <0.05, ** P <0.01, and *** P <0.001 vs Con group; # P <0.05, ## P <0.01, and ### P <0.001. Analysis of variance and Dunnett’s analysis were included to compare the averages of multiple groups. Abbreviations: MNPs, magnetic nanoparticles; AT, actein; NSCLC, non-small-cell lung cancer; Con, control; NS, not significant.

Article Snippet: Rabbit anti-caspase 9 , 1:1,000 , Abcam (ab32539).

Techniques: Activation Assay, Western Blot

Journal: International Journal of Nanomedicine

Article Title: Iron oxide magnetic nanoparticles combined with actein suppress non-small-cell lung cancer growth in a p53-dependent manner

doi: 10.2147/IJN.S127549

Figure Lengend Snippet: Working model of the effect of Fe 3 O 4 magnetic nanoparticle and actein combination on NSCLC cells. Notes: Fe 3 O 4 magnetic nanoparticle and actein combination treatment induces apoptosis in NSCLC cells in a p53-dependent manner. p53 activation results in TRAIL and its DR4 and DR5 contributing to caspase 8 activity through FADD stimulation. Further, p53 activity reduces Bcl2 and BclXL expression, and promotes Bax and Bad expression levels. Therefore, the ratio of Bax:Bcl2 is increased, which leads to activation of caspase 9 and caspase 3. Consequently, apoptosis in NSCLC cells is induced, inhibiting NSCLC development. Abbreviation: NSCLC, non-small-cell lung cancer.

Article Snippet: Rabbit anti-caspase 9 , 1:1,000 , Abcam (ab32539).

Techniques: Activation Assay, Activity Assay, Expressing

Journal: Molecular Medicine Reports

Article Title: Heme oxygenase 1‑overexpressing bone marrow mesenchymal stem cell‑derived exosomes suppress interleukin‑1 beta‑induced apoptosis and aging of nucleus pulposus cells

doi: 10.3892/mmr.2025.13481

Figure Lengend Snippet: Activation of the NF-κB signalling in NP cells induced by IL-1β was repressed after Exo-HO-1 treatment. Western blotting was performed to detect p-p65 and/or p65 protein levels in NP cells with different treatments (control, IL-1β, IL-1β + Exo and IL-1β + Exo-HO-1) as well as in the nuclear (N) and cytoplasmic (C) fractions of the cells. Histone H3 was used as an internal reference for the nucleus. ***P<0.001 vs. control; # P<0.05, ## P<0.01 and ### P<0.001 vs. IL-1β. NP, nucleus pulposus; IL, interleukin; Exo, exosomes; HO-1, heme oxygenase 1; p-phosphorylated.

Article Snippet: After blocking with a rapid blocking solution (Beyotime Institute of Biotechnology) at 4°C overnight, the membranes were incubated with appropriate primary antibodies including anti-HO-1 (cat. no. GTX637432; 1:2,000; GeneTex, Inc.), anti-TGS101 (cat. no. A01233-2, 0.25 μg/ml; Boster Bio), anti-CD63 (FNab01490; 1:1,000; Fine Biotech Co., Ltd, Wuhan, China) and anti-Cainexin (cat. no. GTX109669; 1:5,000; GeneTex, Inc.), Bcl-2 (cat. no. FNab00839; 1:1,000; Wuhan Fine Biotech Co., Ltd.), Bax (cat. no. FNab00810; 1:2,000; Wuhan Fine Biotech Co., Ltd.), cleaved caspase 3 (cat. no. MBS9410752; 1:1,000; MyBioSource, Inc.), anti-p65 (cat. no. PB9324; 0.5 μg/ml; Boster Bio), anti-p-p65 (cat. no. A00284S468-2; 1:2,000; Boster Bio), anti-GAPDH (cat. no. H00227; 1:5,000; Boster Bio) and anti-Histone H3 (cat. no. M12477-9; 1:1,000; Boster Bio) antibodies.

Techniques: Activation Assay, Western Blot, Control

Journal: Translational Cancer Research

Article Title: Extracellular vesicles isolated from curcumin-medium weakened RKO cell proliferation and migration

doi: 10.21037/tcr-24-98

Figure Lengend Snippet: Curcumin suppressed the expression of NF-κB p65 in RKO EVs. The expression of NF-κB p65 in RKO EVs, Cur1-RKO EVs and Cur10-RKO EVs was analyzed by western blotting, respectively. The data are presented as mean ± SD (*, P<0.05). EVs, extracellular vesicles; Cur, curcumin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ns, no significance; SD, standard deviation.

Article Snippet: Polyclonal antibodies against proliferating cell nuclear antigen (PCNA; HY-P80268, 1:4,000), Bcl-2-related X protein (Bax) (HY-P80028, 1:4,000), NF-κB p65 (HY-P80765, 1:1,000), and Calnexin (HY-P80578, 1:1,000) were obtained from MedChemExpress, Shanghai, China.

Techniques: Expressing, Western Blot, Standard Deviation